Ignatov, I., Mosin, O.V. (2015) Methods for Research of Mountain

and Melt Water as Factor of Longevity.

Chemical Composition, NES and DNES Methods for Spectral Analysis.

Effects of Calcium, Magnesium, Zinc and Manganese,

Advances in Physics Theories and Applications, Vol. 44, pp. 48-64.

# Research of Water Samples from Prof. Ignat Ignatov

Research of melt water from Glacier Rosenlaui, Swiss Alps, Prof. Ignatov with collaboration of Eng. Bauer, 2015 |
Research of melt water from Glaciers, Chilean Andes, Prof. Ignatov with collaboration of Pablo Bianchini, 2016 |

## Applied methods

### 1. First method:

Laboratory of Eurotest Control, Sofia, Bulgaria. Decree No. 9 for research of water – chemical composition, hardness, pH. There are including 32-33 indicators with standards ISO 10304-1:2009; ISO 10523:2008; ISO 11885:2009. Additionally are including – bicarbonate ions, hydro carbonate ions, potassium and mercury. Decree No. 9 is for the research of drinking and spring mountain water. Decree No. 14 is for the research of mineral water.

* “The modern science is of the level to take all minerals from mountain and mineral water. If we put these minerals in deionized water we have not mineral or mountain water.” *Prof. Marin Marinov

### 2. Second method:

To understand the “language” of the water there is measurement of the spectrum of water. These results show with local extremums the effects of all minerals and other conditions. Тhe measurements are with two methods (Nonequilibrium Energy Spectrum) NES and (Differential Nonequilibrium Energy Spectrum) DNES. (Prof. Antonov, 1990; Prof. Ignatov, 1998). The standard of study is accepted in peer review journals in USA, European Union and Russia with impact factor.

A convenient method for studying of liquids is non-equilibrium differential spectrum. It was established experimentally that the process of evaporation of water drops, the wetting angle θ decreases discreetly to zero, and the diameter of the water drop basis is only slightly altered, that is a new physical effect (Antonov, 1995; Antonov & Yuskesselieva, 1983). Based on this effect, by means of the measurement of the wetting angle within equal intervals of time is determined the function of distribution of H_{2}O molecules according to the value of f(θ). The distribution function is denoted as the energy spectrum of the water state. The theoretical research established the dependence between the surface tension of water and the energy of hydrogen bonds among individual H_{2}O-molecules (Antonov, 1995).

For calculation of the function f(E) represented the energy spectrum of water, the experimental dependence between the wetting angle (θ) and the energy of hydrogen bonds (E) is established:

where b = 14.33 eV^{-1}

The relation between the wetting angle (θ) and the energy (E) of the hydrogen bonds between H_{2}O molecules is calculated by the formula:

The energy spectrum of water is characterized by a non-equilibrium process of water droplets evaporation, therefore, the term non-equilibrium spectrum (NES) of water is used.

The difference *∆f(E) = f (Esamples of water) – f (Econtrol sample of water)* – is called the “differential non-equilibrium energy spectrum of water” (DNES).

Thus, the DNES spectrum is an indicator of structural changes in water, because the energy of hydrogen bonds in water samples differ due to the different number of hydrogen bonds in water samples, which may result from the fact that different waters have different structures and composition and various intermolecular interactions – various associative elements etc (Ignatov et al, 2014; Ignatov et al., 2015). The redistribution of H_{2}O molecules in water samples according to the energy is a statistical process of dynamics.

Figure 1 shows the average NES-spectrum of deionised water. On the X-axis are depicted three scales. The energies of hydrogen bonds among H_{2}O molecules are calculated in eV. On the Y-axis is depicted the function of distribution of H_{2}O molecules according to energies f(E), measured in reciprocal unit eV^{-1}.
Arrow A designates the energy of hydrogen bonds among H_{2}O molecules, which is accepted as most reliable in spectroscopy.
Arrow B designates the energy of hydrogen bonds among H_{2}O molecules the value of which is calculated as:

Arrow C designates the energy at which the thermal radiation of the human body, considered like an absolute black body (ABB) with a temperature +36.6 0С, is at its maximum.

The figure 1 shows also and the local extremums in water

Figure 1. Nonequilibrium Energy Spectrum (NES) of water

**Notes:**

E=-0.1212 eV is the local extremum for the effects on the nervous system

E=-0.1212 eV is the local extremum for anti-inflammatory effect

E= -0.1387 eV is the local extremum for inhibition of the development of tumor cells of molecular level

### 3. Third method:

Mathematical model of distribution of water molecules according energies of hydrogen bonds (Prof. Ignatov, Ass. Prof. Mosin, 2012)

For example the research with NES method of water drops received after 3 days stay with shungite and zeolite in deionized water may also give valuable information on the possible number of hydrogen bonds as percent of water molecules with different values of distribution of energies (Table 1). These distributions are basically connected with restructuring of H_{2}O molecules with the same energies. The standard of study is accepted in peer review journals in USA, European Union and Russia with impact factor.

Table 1. Mathematical models of water samples with shungite and zeolite

**There is research with DNES method. The sample is with 1% solution of food supplements, drugs, chemical compositions and chemical elements. The result is with NES method The control sample is with deionized water and the results is with NES method. The difference between NES of 1% solution and NES of deionized water gives DNES.**

**Results of 1% (v/v) solution in deionized water of VITA intense**

The research with the NES method of water drops is received with 1% solution VITA intense, and deionized water as control sample. The mathematical models of 1% (v/v) solution VITA intense gives the valuable information for the possible number of hydrogen bonds as percent of H_{2}O molecules with different values of distribution of energies (Table 2 and Fig. 2). These distributions are basically connected with the restructuring of H_{2}O molecules having the same energies.

Table 2: The distribution (%, (-Evalue)/(-Etotal value) of H

_{2}O molecules in 1% water solution of VITA intense (product of LavaVitae, Austria) and control deionized water

E= -0.1112 eV is the local extremum for relaxing effect on nervous system

E=-0.1212 eV is the local extremum for anti-inflammatory effect

E= -0.1387 eV is the local extremum for inhibition of development of tumor cells of molecular level

Notes:

* The result (-Evalue) is the result of hydrogen bonds energy for one parameter of (-E)

** The result (-Etotal value) is the total result of hydrogen bonds energy

Figure 2 shows the distribution (%, (-Evalue)/(-Etotal value) of H_{2}O molecules in and 1% (v/v) of water solution of VITA intense (product of LavaVitae, Austria) (red line) and control sample deionized water (blue line).

Figure 2: Mathematical model (Ignatov, Mosin, 2013) of 1% water solution of VITA intense (product of LavaVitae, Austria)

Notes:

E= -0.1112 eV is the local extremum for relaxing effect on nervous system

E=-0.1212 eV is the local extremum for anti-inflammatory effect

E= -0.1387 eV is the local extremum for inhibition of development of tumor cells of molecular level

### 4. Forth method: Results with pH and ORP

There are valid the following results of pH as indicator for acid alkaline medium of the products of LavaVitae. There are the results also of ORP or Oxidation-reduction potential.

The results are for 1% (v/v) of solutions of products, which are made from deionized water. This research is performed with Ass. Prof. Georgi Gluhchev from Bulgarian Academy of Science (BAS). The results of pH of deionized water is 6.05 and of ORP is 119.7. Table 3 shows the results of pH and ORP.

Table 3. Results of products of company LavaVitae for pH and ORP

Figure 3 shows the dependence between the acidity and basicity (pH) of electrochemically activated solutions and the oxidation-reduction potential (ORP). The pH value within the interval from 3 to 10 units and the ORP within the interval from -400 mV to +900 mV characterize the area of the biosphere of microorganisms. Outside these ranges of pH and ORP the microorganisms will hardly survive.

Figure 3: The dependence between acidity and basicity (pH) of solutions and the ORP on the biosphere of micro-organisms (point 1; VITA intense), (point 2; BOOST), point 3; ZEOLITH detox).

The result of 1% (v/v) solution of VITA intense is 4.07 or acidic medium. The result of ORP is (-104.5). The result of ORP with negative charge is connected with charge with negative value, which has antioxidant and permanent antioxidant activity. In the VITA intense there are the following antioxidants – Vitamins C, E, D. Figure 11 shows the dependence between acidity and basicity (pH) of solutions and the ORP on the biosphere of micro-organisms. The result of VITA intense with point 1 with coordinates (4,07; -104.5) is the biosphere of micro-organisms. VITA intense is useful for human health also with liquid form.

### Conclusions and table with total results. Biophysical and biochemical effects

From the results with methods 1. 2. and 3 there is structuring of table with results and conclusions with biophysical and biochemical effects on human body.

Table 2. shows optimal chemical composition of water, hardness, local extremum eV^{-1} at (-0.1362–-0.1387 eV) and total mineralization of water

The results of table 2 show antioxidant effects

For contacts – **mbioph@dir.bg**

The dose for research is 2 liters. The technical time for the study is 3 weeks.

CV of Prof. Ignat Ignatov: http://www.medicalbiophysics.bg/en/ignat_ignatov.html

Google Scholar: https://scholar.google.bg/citations?user=UHnsf3MAAAAJ&hl=de

### Five publications of Prof. Ignatov on the topic water with impact factor more than 30.

1. Ignatov I., Mosin O.V. (2013) Possible Processes for Origin of Life
and Living Matter with Modeling of Physiological Processes of Bacterium Bacillus
Subtilis in Heavy Water as Model System, Journal of Natural Sciences Research,
Vol. 3, No. 9, pp. 65-76. Impact factor 5.53

2. Ignatov, I., Mosin, O. V. (2013) Structural Mathematical Models
Describing Water Clusters, Journal of Mathematical Theory and Modeling,
Vol. 3, No. 11, pp. 72-87. Impact factor 5.58

3. Ignatov, I., Mosin, O. V. (2014) The Structure and Composition of Carbonaceous Fullerene Containing Mineral Shungite and Microporous Crystalline Aluminosilicate Mineral Zeolite. Mathematical Model of Interaction of Shungite and Zeolite with Water Molecules, Advances in Physics Theories and Applications, Vol. 28, pp. 10-21. Impact factor 7.17

4. Ignatov, I., Mosin, O. V., Velikov, B., Bauer, E. Tyminski, G. (2014) Longevity
Factors and Mountain Water as Factor. Research in Mountain and Fields Areas
in Bulgaria, Civil and Environmental Research, Vol. 30, No. 4, pp. 51-60. Impact factor 5.58

5. Ignatov, I., Mosin, O.V., Kirov, P. (2016) Mathematical Model of Kangen Water®. Biophysical and Biochemical Effects of Catholyte, Advances in Physics Theories and Applications, Vol. 51, pp. 33-55. Impact factor 7.17

**The total impact factor of 5 publications of Prof. Ignatov is 31.03**