This article outlines the results on the antiviral and antimicrobial action of electrolytic water solutions of sodium chloride (anolyte/catholyte), produced in the anode and cathode chamber of the electrolytic cell, on the classical swine fever (CSF) virus and E. coli DH5. The anolyte did not affect the growth of the cell culture PK-15; the viral growth during the infection of a cell monolayer with a cell culture virus was affected in the greatest degree by the anolyte in 1:1 dilution and less in other dilutions; whereas the viral growth at the infection of a cell suspension with the CSF virus was affected by the anolyte in dilution 1:1 in the greatest degree, and less by other dilutions; the viral growth at the infection with the CSF virus in suspension of the cell monolayer was affected by the anolyte in all dilutions. Unexpectedly, the stronger biocidal effect of the catholyte was observed when a strain of E. coli DH5 was treated by the anolyte and catholyte, respectively. In order to provide additional data about the antimicrobial and antiviral activity of the electrochemically activated solutions of NaCl and the distribution of H2O molecules according to the energies of hydrogen bonds, the non-equilibrium energy spectrum (NES) and differential non-equilibrium energy spectrum (DNES) of the anolyte and the catholyte were measured.

Keywords: anolyte, catholyte, E. coli DH5, CSF virus, NES, DNES