Studies were carried out to determine the sensitivity of pathogenic Gram-negative bacteria (Salmonella enterica ATCC and Pseudomonas aeruginosa strain №450) to electrochemically activated 3% aqueous sodium chloride solution (anolyte) in concentrations of 100%, 50%, 25% and 12,5% immediately after preparation (fresh) and after storage at room temperature for 7 days, 21 days and 28 days. As a control was used the disinfectant Virkon S in concentrations of 1%, 0,5%, 0,25% and 0,125%. It was found that the suspension of P. aeruginosa with density of 10⁶ cells/ml was killed after 2 minutes under the influence of all tested concentrations of the fresh anolyte. The same strain in concentration of 10⁸ cells/ml was killed within 2 minutes by 50% and 100% fresh anolyte, but the lower concentrations had such effect after 5 - 10 minutes. The anolyte storaged 7 days had similar action. After 21 and 28 days the anolyte in all tested concentrations had fast bactericidal activity on P. aeruginosa in suspension of 10⁶ cells/ml (in 2 minutes), but in such of 10⁸ cells/ml single cells remained viable even after 10 minutes. S. enterica in both tested suspensions - of 10⁶ and 10⁸ cells/ml was inactivated within 2 minutes by all examined concentrations not only of the fresh anolyte, but of these stored for 7, 21 and 28 days. Virkon S in all tested concentrations killed within two minutes P. aeruginosa and S. enterica in both examined densities.
Keywords: electrochemically, sodium chloride, anolyte, Pseudomonas aeruginosa, Salmonella enterica, antibacterial activity.