The aim of the study was to analyze nano and microbiological effects of EVODROP Silver and Copper Nanoparticle. Studies of the nanotechnological material Silver Nanoparticle and EVODROP Copper Nanoparticle with author Fabio Huether have been performed. EVODROP Silver and Copper Nanoparticle was dissolved in water with a concentration of 30 ppm. The same concentration of 30 ppm was of Silver Nanoparticle. Two research methods were used. The comparative analyses were performed with the method of Differential Nonequilibrium Spectrum (DNES) between Colloidal Nano Silver Nanoparticle and EVODROP Silver and Copper Nanoparticle. Microbiological analyses were applied for the effects of EVODROP Silver and Copper Nanoparticle on the bacteria in water. The bacteria are – Escherichia coli, Enterococci and Coliforms. The authors recommend EVODROP Silver and Copper Nanoparticle for surface disinfection and sprays with antibacterial and antiviral activity.