Research of deionized water after filtration with shungite was performed. For the research of physicochemical parameters, the filtration lasted 24 hours for 50 g shungite in 1 L deionized water with conductivity 3.13 µS cm^-1. For in vitro studies of microbiological parameters, the effect of shungite was studied for 12 and 36 hours. The research was performed with Staphylococcus aureus and Escherichia coli. The shungite was received from Russian Federation with Material Safety Data Sheet and Certificate of conformity. The study was executed according to Ordinance No. 9/2001 with determination of 31 physicochemical parameters and additionally of potassium (K), hydrocarbonates (HCО₃^-) and carbonates (CО₃^-). After filtration with shungite, all 34 parameters were in norm and the water was suitable for drinking. The pH of shungite deionized water was 6.30. The pH of deionized water before the research was 6.09. The study proved that deionized water after filtration through shungite is not saturated with minerals of greater value than the normal one. Analyzes for the filtering properties of shungite are shown. The antibacterial effect of shungite water (aqueous infusions of shungite) obtained for 12 and 36 hours was tested. Staphylococcus aureus-ATCC and TSA-MRSA and Escherichia coli ATCC strains were used in the studies. Both tested aqueous infusions of shungite reduced the amount of viable E. coli and S. aureus cells even when they were in high concentrations (106 cells mL^-1). The effect of the 36-hour infusion was better, under the influence of which after 15 min the number of live bacteria from the two tested species decreased to 85.5 + 5.0 %. After 120 min, the 12-hour infusion completely inactivated E. coli cells and reduced those of S. aureus to 38.8 + 7.4%. However, about 25 % of S. aureus cells survived after 24 hours of exposure to both aqueous infusions of shungite, and 20 % of them - even after 96 hours of exposure to these infusions. The antibacterial effect of the two aqueous infusions of shungite was established. These results show prospects for the use of shungite water as a prophylactic and adjuvant treatment for bacterial gastroenteritis and other infections.
Keywords: shungite water, nano, physicochemical parameters, antibacterial activity