Studies have been carried out to determine the effect of activated aqueous solutions (anolytes and catholytes) on multi-resistant strain of Pseudomonas aeruginosa in vitro after storage at room temperature. The solutions were obtained by electrochemical activation of water with 0.8% NaCl, and with a combination of 0.4% NaCl and 0.4% Na2CO3. The disinfectant Virkon S was used as a control. Cultures were made on Cetrimide agar after different intervals of action of the solutions on P. aeruginosa suspensions (106 cells/ml). Anolytes and catholytes tested exhibited high antimicrobial activity. Upon potentiation of the anolytes by addition of 96% ethanol with a final concentration of 1%, bactericidal activity was observed within 2 minutes. The use of catholyte of NaCl to dissolve Virkon S resulted in an increase in its effect compared to its use as an aqueous solution. After storage for 29 days, the solutions tested preserved their antibacterial properties to the maximum extent.
Keywords: Antibacterial activity, Anolyte, Catholyte, Pseudomonas aeruginosa